CIL:36713, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus, cortical microtubule cytoskeleton; Biological process:microtubule cytoskeleton organization, oral apparatus organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36713^* Cite

Description

The postoral fibers are also heavily labeled by anti-b-tubulin. The cell was lightly fixed, placed in metylcellulose and frozen in liquid nitrogen. Sections were produced at -100°C before thawing and treated with the b-tubulin antibody followed by immunogold labeled antibody to IgG. TEM taken on 2/25/94 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: oral apparatus; cortical microtubule cytoskeleton

Biological Context

Biological Process: microtubule cytoskeleton organization; oral apparatus organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36713
Archival Resource Key (ARK): ark:/b7295/w9cil36713

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: intrinsic mass distribution; stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; gold
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2130px	——
Y	2178px	——