CIL:36710, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Alternate header for print version

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:transport vesicle, microtubule associated complex, cytoplasmic dynein complex; Biological process:cytoplasmic microtubule organization, vesicle docking involved in exocytosis;

Open Detailed Viewer

Licensing

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36710^* Cite

Description

Details of the binding of the carrier vesicle to a microtubule. Four bridges, thought to be cytoplasmic dynein arms, span the 40nm space between these two structures. The membrane of the vesicle has been fractured to expose its smooth E-face. TEM taken on 5/18/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.1µm. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:40507) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/). Published in J. Cell Biol. 111:2553-2562, 1990. Adapted with permission.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: transport vesicle; microtubule associated complex; cytoplasmic dynein complex

Biological Context

Biological Process: cytoplasmic microtubule organization; vesicle docking involved in exocytosis

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Published: J Cell Biol. 1990 Dec;111(6 Pt 1):2553-62.
Pubmed: 2148940

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36710
Archival Resource Key (ARK): ark:/b7295/w9cil36710

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2944px	——
Y	3744px	——