CIL:36695, Danio rerio, embryo. CIL. Dataset

NCBI Organism:Danio rerio; Cell Components:microtubule, , actin cytoskeleton; Biological process:mitosis

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36695^* Cite

Description

Confocal z-stack of a dividing zebrafish embryo. The fixed embryo is visualized using EMTB-3GFP (microtubules-marker - green), phalloidin (actin marker -red) and Hoechst (DNA marker - blue).

Technical Details

Images collected on a Zeiss LSM 710 confocal with a 20X 0.8 NA objective using 561, 633, and 750 nm laser lines.

Biological Sources

NCBI Organism Classification: Danio rerio
Cell Type: embryo
Cellular Component: microtubule; DNA; actin cytoskeleton

Biological Context

Biological Process: mitosis

Attribution

Names: Martin Wuehr; Timothy Mitchison

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36695
Archival Resource Key (ARK): ark:/b7295/w9cil36695

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; distribution of a specific protein
Visualization Methods: labeled primary antibody; phalloidin; hoechst
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2048px	0.3459µm
Y	2048px	0.3459µm