CIL:36683, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:trichocyst, ; Biological process:organelle organization, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36683^* Cite

Description

Electric shock causes massive trichocyst discharge and results in a large accumulation of HRP-labeled vesicles at the ectoplasmic/endoplasmic interface (at the level of the internal ends of the undischarged trichocysts. TEM taken on 4/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,750X. Bar = 0.25µm.

Technical Details

Electric shock and HRP-labeling followed by standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: trichocyst; cytoplasm

Biological Context

Biological Process: organelle organization; organelle discharge

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36683
Archival Resource Key (ARK): ark:/b7295/w9cil36683

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: intrinsic mass distribution; stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; gold
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2568px	——
Y	2106px	——