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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36662*  Cite 
Description

In a 9-day old culture of stationary phase cells, two or three days post-conjugation, remnants of the previous macronucleus could be found near normal macronuclear bodies. This remnant was identified by its nuclear envelope and a nucleolar body. A sparsely dispersed array of other electron-opaque bodies was present but these were far fewer than in a normal macronucleus. Presumably this body represents a breakdown product of the disintegrating macronucleus that was present before the preceding conjugation that occurred on day 6 or 7. TEM taken on 3/6/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,500X. Bar = 1µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
nucleolus
macronucleus
Biological Context
Biological Process
nucleolus organization
macronucleus organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36662
Archival Resource Key (ARK)
ark:/b7295/w9cil36662
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2642px ——
Y 3000px ——