CIL:36652, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:micronucleus, microtubule; Biological process:nuclear division

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36652^* Cite

Technical Details

QF-DE of the separation spindle of a micronucleus apparently near a daughter micronucleus (not in section plane). The spindle is filled with microtubules linked together by bridges or by motor proteins. An intact nuclear envelope with pores encloses the spindle. TEM taken on 5/12/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: micronucleus; microtubule

Biological Context

Biological Process: nuclear division

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36652
Archival Resource Key (ARK): ark:/b7295/w9cil36652

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3032px	——
Y	3829px	——