CIL:36648, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:nuclear pore, nuclear membrane; Biological process:nuclear pore distribution, nuclear membrane organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36648^* Cite

Description

A freeze-fractured view of the nuclear envelope. The fracture in the middle of the figure exposes the cytosolic facing membrane. This can be distinguished by a fractured cytoplasmic organelle at the top of the picture. The lower part of the picture shows the nucleoplasm-facing membrane of the envelope. The nuclear pores penetrate through both membranes. It is not clear if the membranes have actually fractured along their hydrophobic centers or if the true surfaces of these two membranes are being viewed. TEM taken on 2/16/76 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,579X. Bar = 0.25µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: nuclear pore; nuclear membrane

Biological Context

Biological Process: nuclear pore distribution; nuclear membrane organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36648
Archival Resource Key (ARK): ark:/b7295/w9cil36648

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2323px	——
Y	3000px	——