CIL:36639, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:peroxisome, smooth endoplasmic reticulum, microtubule associated complex, mitochondrion; Biological process:cytoplasm organization, organelle organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36639^* Cite

Description

In quick-freeze deep-etch micrographs it is not easy to distinguish peroxisomes from surrounding organelles. The organelle at the top of this micrograph may be a peroxisome based on its finely granular content. Also the elongated vesicle showing a porous E-fracture face in the middle may also be a peroxisome. Several mitochondria and the porous E-face of the smooth spongiome of the Contractile Vacuole Ccomplex (associated with a microtubular ribbon) are present. TEM taken on 6/22/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 15,900X. Bar = 0.25µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: peroxisome; smooth endoplasmic reticulum; microtubule associated complex; mitochondrion

Biological Context

Biological Process: cytoplasm organization; organelle organization

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36639
Archival Resource Key (ARK): ark:/b7295/w9cil36639

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2442px	——
Y	3000px	——