CIL:36628, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:endoplasmic reticulum Biological process:endoplasmic reticulum organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36628^* Cite

Description

A view of the ER on which IMPs are organized into lines, arcs, and circles indicating organization of proteins within the ER membrane and possibly peripheral proteins attached to its cytosolic surface. TEM taken on 3/4/97 by R. Allen with Zeiss 10A operating at 80kV. Neg. 15,900X. Bar = 0.5µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: endoplasmic reticulum

Biological Context

Biological Process: endoplasmic reticulum organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36628
Archival Resource Key (ARK): ark:/b7295/w9cil36628

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2549px	——
Y	3000px	——