CIL:36615, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, , trichocyst, cilium, cytoplasm; Biological process:cortical cytoskeleton organization, cytoplasm organization;

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36615^* Cite

Description

The infraciliary lattice in this quick-freeze deep-etched image consists of a meshwork of branching fibers composed of many subfibers. Basal bodies and trichocyst tips extend through the openings of the mesh. Individual metal-coated fibrils of the mesh average 11 nm in diameter and are closely interlaced and cross-linked to other fibrils. TEM taken on 5/27/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm. There is a high resolution version of this image in the library (CIL:39319) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; mitochondria; trichocyst; cilium; cytoplasm

Biological Context

Biological Process: cortical cytoskeleton organization; cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36615
Archival Resource Key (ARK): ark:/b7295/w9cil36615

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2154px	——
Y	2339px	——