CIL:36613, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:plasma membrane, cilium, trichocyst, ; Biological process:plasma membrane organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36613^* Cite

Description

The P-face of the ciliary membrane at the base of the cilium has 9 triple rows of IMPs in register with the 9 doublets of the internal axoneme. It is thought that these IMPs may be sites of calcium influx into the base of the cilium for regulating ciliary activity. Also, trichocyst docking sites. TEM taken on 1/6/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39317) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: plasma membrane; cilium; trichocyst; trichocyst docking sites

Biological Context

Biological Process: plasma membrane organization

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36613
Archival Resource Key (ARK): ark:/b7295/w9cil36613

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3166px	——
Y	2656px	——