CIL:36610, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:plasma membrane, cilium axoneme; Biological process:plasma membrane organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36610^* Cite

Description

In this image a portion of the true surface of the plasma membrane is exposed where the culture medium has been etched away. The IMPs of the plates can also be viewed less distinctly on the true surface of the cell. The edge of the fractured and removed E leaflet is visible. Fractured doublets of the axoneme of a cilium lie nearby. TEM taken on 6/4/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2 µm. There is a high resolution version of this image in the library (CIL:39315) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: plasma membrane; cilium axoneme

Biological Context

Biological Process: plasma membrane organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36610
Archival Resource Key (ARK): ark:/b7295/w9cil36610

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3142px	——
Y	3456px	——