CIL:36607, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:plasma membrane Biological process:plasma membrane organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36607^* Cite

Description

In quick-freeze deep-etch preparations the plasma membrane can be seen to contain plaques or plates of intramembrane particles (IMPs) that are regularly aligned in rows with a center-to-center spacing between rows of 25nm. These plaques remain in the P-fracture face of the plasma membrane and are found mostly on the anterior ventral surface of the cell near the anterior suture. Their function is not known. For discussion see Allen, J. Ultrastruc. Res. 63:64-78, 1978 and Hufnagel, Micros. Res. Tech. 22:225-264, 1992. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. There is a high resolution version of this image in the library (CIL:39314) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: plasma membrane

Biological Context

Biological Process: plasma membrane organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36607
Archival Resource Key (ARK): ark:/b7295/w9cil36607

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2148px	——
Y	2675px	——