CIL:36601, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:pellicle, cell cortex; Biological process:plasma membrane organization, cortical cytoskeleton organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36601^* Cite

Description

This view shows a transverse ridge cut with its sloping side lying tangentially in the section plane. The granulo-fibrillar filled peaks are prominent. Striated bands appear sectioned closer to their mid-sections, probably in areas of filament overlap. The outer alveolar membrane is not usually continuous with the inner alveolar membrane at the tips of the ridges. TEM taken on 11/11/70 by R. Allen with Hitachi HU11A operating at 60kV. Neg. 37,700X. Bar = 0.1µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39311) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: pellicle; cell cortex

Biological Context

Biological Process: plasma membrane organization; cortical cytoskeleton organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36601
Archival Resource Key (ARK): ark:/b7295/w9cil36601

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2368px	——
Y	2283px	——