CIL:36542, Xenopus laevis, fertilized egg. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Components:microtubule, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36542^* Cite

Description

This image is part of a large data set of Xenopus laevis eggs imaged at various times post fertilization (the first number of the file name corresponds minutes, eg 30_2 is 30 min post fertilization). The embryos are stained for alpha-tubulin (green) using a directly labeled B-5-1-2 antibody and DNA (red).

Technical Details

Images collected on a Zeiss LSM 710 confocal with a 10X 0.3 NA objective using 561 and 633 laser lines.

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: fertilized egg
Cellular Component: microtubule; DNA

Biological Context

Biological Process

Attribution

Names: Martin Wuehr; Timothy Mitchison

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36542
Archival Resource Key (ARK): ark:/b7295/w9cil36542

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; distribution of a specific protein
Visualization Methods: labeled primary antibody
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2592px	0.48µm
Y	2592px	0.48µm