CIL:36266, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Vorticella convallaria; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:Golgi transport complex, food vacuole; Biological process:post-Golgi vesicle-mediated transport, Golgi to vacuole transport;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36266^* Cite

Description

Accumulation of golgi cupulas near a food vacuole. The food vacuole shown has already fused with many vesicles that have already added their membrane as well as their dense inner lining to the vacuole lumen. TEM taken on 2/17/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39458) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Vorticella convallaria
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component: Golgi transport complex; food vacuole

Biological Context

Biological Process: post-Golgi vesicle-mediated transport; Golgi to vacuole transport

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36266
Archival Resource Key (ARK): ark:/b7295/w9cil36266

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3062px	——
Y	3313px	——