CIL:36257, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Vorticella convallaria; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:pellicle, cell cortex, coated pit, clathrin coat, rough endoplasmic reticulum membrane; Biological process:clathrin coat assembly, vesicle targeting, rough ER to cis-Golgi;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36257^* Cite

Description

The pellicular pore consists of the invaginated plasma membrane that penetrates the alveolus as well as the epiplasm and ends as a clathrin-coated pit. TEM taken on 3/14/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 23,500X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39324) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Vorticella convallaria
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component: pellicle; cell cortex; coated pit; clathrin coat; rough endoplasmic reticulum membrane

Biological Context

Biological Process: clathrin coat assembly; vesicle targeting, rough ER to cis-Golgi

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36257
Archival Resource Key (ARK): ark:/b7295/w9cil36257

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3376px	——
Y	2837px	——