CIL:36242, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Tetrahymena pyriformis; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:microtubule basal body, microtubule associated complex, fibril; Biological process:microtubule basal body duplication

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36242^* Cite

Description

During division new basal bodies develop accessory fibers and begin to move apart to take up a position equidistant between the older complexes. The new kinetodesmal fiber seems to be associated with the prexisting kinetodesmal fiber during separation. TEM taken on 8/15/67 by R. Allen with Philips 200 operating at 60kV. Neg. 12,400X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39798) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: microtubule basal body; microtubule associated complex; fibril

Biological Context

Biological Process: microtubule basal body duplication

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36242
Archival Resource Key (ARK): ark:/b7295/w9cil36242

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3143px	——
Y	3636px	——