CIL:36216, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Tetrahymena pyriformis; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:microtubule basal body, microtubule associated complex, mitochondrion, pellicle; Biological process:cortical cytoskeleton organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36216^* Cite

Description

A post ciliary microtubular ribbon extends from the proximal left side of the basal body and arches toward the cell surface where it ends near the longitudinal microtubular ribbon at the crest of the pellicular ridge. TEM taken on 8/6/65 by R. Allen on RCA EMU3F operating at 50kV. Neg. 18,400X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: microtubule basal body; microtubule associated complex; mitochondrion; pellicle

Biological Context

Biological Process: cortical cytoskeleton organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36216
Archival Resource Key (ARK): ark:/b7295/w9cil36216

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: intrinsic mass distribution; stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; gold
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3056px	——
Y	3128px	——