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NCBI Organism:Homo sapiens; Cell Types:fibroblast Cell Components:actin filament bundle, stress fiber; Biological process:actin cytoskeleton organization
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36074*  Cite 
Description

A human skin fibroblast in vitro stained by indirect immunofluorescence with an antibody to tropomyosin. The pattern of tropomyosin staining corresponds with stress fibers and the distribution of polymerized actin. Image from Elias Lazarides, J. Cell Biol. 65:549-61, 1975, PMID: 1094020. Figure 444 from Chapter 16 (Cytoplasmic matrix and cytoskeleton) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB’s BioEDUCATE website.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cellular Component
actin filament bundle
stress fiber
Biological Context
Biological Process
actin cytoskeleton organization
Molecular Function
tropomyosin binding
Attribution
Names
Don W. Fawcett
Elias Lazarides
Published
ISBN 0721635849
Link
http://www.ascb.org/bioeducate/FawcettTheCell...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36074
Archival Resource Key (ARK)
ark:/b7295/w9cil36074
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Data Qualifiers
processed data
Sample Preparation
Methods
crosslinking-fixative fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 888px ——
Y 1230px ——