CIL:36032, Homo sapiens, epithelial cell. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:epithelial cell Cell Components:nuclear pore Biological process:nuclear pore complex assembly, nucleus organization;

Licensing

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CIL:36032^* Cite

Description

HeLa cells were processed for indirect immunofluorescence with antibodies to two nuclear pore components, ELYS (embryonic large protein derived from yolk sac) and mAb414 against the FG nucleoporins. Both co-localize in a punctate manner to the nuclear envelope as expected for nuclear pore components. See Fig 2A in Rasala et al. Proc Natl Acad Sci 103:17801-17806.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell
Cell Line: HeLa
Cellular Component: nuclear pore

Biological Context

Biological Process: nuclear pore complex assembly; nucleus organization

Attribution

Names: Beth Rasala; Arturo Orjalo; Zhouxin Shen; Steven Briggs; Douglass Forbes
Published: B Rasala et al. 2006 Proc Natl Acad Sci USA 103:17801-17806
Pubmed: 17098863
Link: nuclear envelope

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36032
Archival Resource Key (ARK): ark:/b7295/w9cil36032

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; 3D-deconvolution
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Processing History: montage
Data Qualifiers: processed data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	516px	——
Y	759px	——