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NCBI Organism:Chlorocebus aethiops; Cell Types:permanent cell line cell Biological process:negative regulation of insulin receptor signaling pathway
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36023*  Cite 
Description

COS7 cells, cotransfected with GFP-PTP1B, APN-EphA3, and TM-BirA, were stimulated with SA Dynabeads. Cell surface EphA3 was labeled on intact cells with IIIA4 α-EphA3 mAb and detected with Alexa Fluor 546–labeled secondary antibodies by confocal microscopy. PTP1B/EphA3 colocalization is apparent around SA Bynabeads.

Biological Sources
NCBI Organism Classification
Chlorocebus aethiops
Cell Type
permanent cell line cell
Cell Line
COS-7
Biological Context
Biological Process
negative regulation of insulin receptor signaling pathway
Molecular Function
protein tyrosine kinase activity
Attribution
Pubmed
PMID: 21135139
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36023
Archival Resource Key (ARK)
ark:/b7295/w9cil36023
Grouping This image is part of a group.
Imaging
Image Type
fluorescence microscopy
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Green fluorescent proteins from Aequorea
Alexa Fluor 546
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px ——
Y 1024px ——