CIL:35875, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:, nucleus, cytoplasm; Biological process:cell cycle, regulation of transcription, DNA-dependent, regulation of cell size, regulation of transcription involved in G1/S phase of mitotic cell cycle;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35875^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged WHI5. WHI5 is a repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1. These phase, gfp images are part of an image group that ranges from CIL:35860-35879. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: WH15 protein; nucleus; cytoplasm

Biological Context

Biological Process: cell cycle; regulation of transcription, DNA-dependent; regulation of cell size; regulation of transcription involved in G1/S phase of mitotic cell cycle
Molecular Function: GO:0016566

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35875
Archival Resource Key (ARK): ark:/b7295/w9cil35875

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm

Time	300 microns	61