CIL:35852, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:, cellular bud neck, spindle pole body; Biological process:cytokinesis, regulation of exit from mitosis, protein phosphorylation, meiotic anaphase II;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35852^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc15, a protein kinase involves in cytokinesis. These phase and GFPimages are part of an image group containing CIL 35840-35859. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

Technical Details

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please read the microscopy section of the referenced paper for details before image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: CDC15 Protein; cellular bud neck; spindle pole body

Biological Context

Biological Process: cytokinesis; regulation of exit from mitosis; protein phosphorylation; meiotic anaphase II
Molecular Function: protein kinase activity

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35852
Archival Resource Key (ARK): ark:/b7295/w9cil35852

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm

Time	300 seconds	61