CIL:35662, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:, pre-replicative complex, DNA replication preinitiation complex; Biological process:G1/S transition of mitotic cell cycle, pre-replicative complex assembly, regulation of chromatin silencing at telomere, regulation of DNA-dependent DNA replication initiation, mannoprotein catabolic process;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35662^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc6. Cdc 6 is an essential ATP-binding protein required for DNA replication, component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p. These phase, gfp images are part of an image group that ranges from CIL:35660-35679. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: CDC6 Protein; pre-replicative complex; DNA replication preinitiation complex

Biological Context

Biological Process: G1/S transition of mitotic cell cycle; pre-replicative complex assembly; regulation of chromatin silencing at telomere; regulation of DNA-dependent DNA replication initiation; mannoprotein catabolic process
Molecular Function: ATP binding; ATPase activity; chromatin binding; DNA replication origin binding; GTP binding; GTPase activity; protein binding

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35662
Archival Resource Key (ARK): ark:/b7295/w9cil35662

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm

Time	300 seconds	61