CIL:35654, Saccharomyces cerevisiae. CIL. Dataset

Cell Components:, mitotic checkpoint complex, anaphase-promoting complex; Biological process:activation of mitotic anaphase-promoting complex activity, mitotic metaphase/anaphase transition, mitotic sister chromatid segregation, mitotic spindle elongation, mannoprotein catabolic process;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35654^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc20, a cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C) required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate novel protein thought to be involved in cell cycle regulation. These phase, gfp images are part of an image group that ranges from CIL:35640-35659. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: CDC20 protein; mitotic checkpoint complex; anaphase-promoting complex

Biological Context

Biological Process: activation of mitotic anaphase-promoting complex activity; mitotic metaphase/anaphase transition; mitotic sister chromatid segregation; mitotic spindle elongation; mannoprotein catabolic process
Molecular Function: protein binding; ubiquitin-protein ligase activity

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35654
Archival Resource Key (ARK): ark:/b7295/w9cil35654

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm

Time	300 seconds	61