CIL:35618, Homo sapiens, keratinocyte. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:keratinocyte Cell Components:actin filament, , , adherens junction; Biological process:, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35618^* Cite

Description

The relationship between the actin cytoskeleton (blue), adherens junctions (green) and phospho-Myosin Light Chain (MLC) (red) after 30 minutes of calcium stimulation.

Technical Details

The specimen was fixed with labelled with 4% paraformaldehyde and labeled with AlexaFluor488 phalloidin, rabbit anti alpha-catenin primary antibodies and Cy5 anti-rabbit secondary antibodies, mouse anti Phospho-MLC Ser18/Thr19 antibodies (Cell Signaling technologies) and mouse anti cy2 secondary antibodies. Images were acquired on a Zeiss LSM510 inverted microscope using a 63x 1.4 Numeric aperture oil immersion objective.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: keratinocyte
Cellular Component: actin filament; myosin light chain; alpha catenin; adherens junction

Biological Context

Biological Process: phosphorylation of myosin light chain; calcium stimulation

Attribution

Names: Ann Wheeler
OTHER: Queen Mary University London; Advanced Light Microscopy Facility

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35618
Archival Resource Key (ARK): ark:/b7295/w9cil35618

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: primary antibody plus labeled secondary antibody; phalloidin; Alexa Fluor 488; Cy2; Cy5
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	740px	0.143µm
Y	1023px	0.143µm