CIL:35617, Xenopus laevis, tissue culture cell. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35617^* Cite

Description

Time lapse movie of mitosis in Xenopus laevis tissue culture cell. The sequence illustrates the requirement for all chromosomes to be aligned on the metaphase plate before anaphase is initiated.

Technical Details

Phase contrast images were captured at 15 sec intervals using a Zeiss 200 inverted microscope with 63x 1.4NA objective lens.

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: tissue culture cell
Cell Line: S3
Cellular Component: nucleus; nuclear chromosome; spindle

Biological Context

Biological Process: mitosis; chromosome organization; nucleus organization

Attribution

Names: John R. Daum; Gary J Gorbsky

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35617
Archival Resource Key (ARK): ark:/b7295/w9cil35617

Imaging

Image Type: charge coupled device (CCD)
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	518px	——
Y	396px	——

Time	15 seconds	55