Alternate header for print version
Logo


NCBI Organism:Homo sapiens; Cell Types:epithelial cell, ; Cell Components:spindle, kinetochore; Biological process:mitosis
Image Data Download Options...
Open Detailed Viewer

Licensing
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35590*  Cite 
Description

Nup133 (a component of the Nup107-160 nucleoporin complex, red) localizes to spindle poles and kinetochores while NuMA localizes to spindle poles (green) in prometaphse Hela cells. See Fig 2D in Orjalo et al. 2006 Mol Biol Cell 17:3806-3818.

Technical Details

Cultured HeLa cells were permeabilized, fixed with methanol at -20C for 10 min and processed for immunofluorescence microscopy and recorded using a Zeiss Axioskop microscope.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
cervical carcinoma
Cell Line
HeLa
Cellular Component
spindle
kinetochore
Biological Context
Biological Process
mitosis
Attribution
Names
Arturo Orjalo
Alexei Arnaoutov
Zhouxin Shen
Yekaterina Boyarchuk
Samantha Zeitlin
Beatriz Fontoura
Steven Briggs
Mary Dasso
Douglass Forbes
Published
Orjalo et al. 2006 The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol Biol Cell 17:3806-3818
Pubmed
16807356
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35590
Archival Resource Key (ARK)
ark:/b7295/w9cil35590
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Processing History
montage
Data Qualifiers
processed data
Sample Preparation
Methods
coagulating-fixative fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1390px ——
Y 473px ——