CIL:35532, Lilium longiflorum, gamete. CIL. Dataset

NCBI Organism:Lilium longiflorum; Cell Types:gamete Cell Components:pollen wall, exine, intine, sexine, endexine; Biological process:pollination

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35532^* Cite

Description

Lilium longiflorum (Easter Lily) pollen. This is a thin optical section through the center of the desiccated stage of the mature pollen showing autofluorescence or harmonic generation of intrinsic structures. The brightest signal is from the outer layer, the exine and raised pilae.

Technical Details

Illumination provided by a SpectraPhysics-Newport MaiTai laser with DeepSee dispersion compensation providing pulses of approximately 100 fs duration at 80 MHz. Wavelength set at 860 nm with emission at 465 to 50 nm. Zeiss 710 multi-photon microscope with 63X N.A. 1.4 oil immersion objective. Pollen was removed from the flower, put in a coverslip bottom chamber, covered with tissue culture grade mineral oil, and imaged within 12 hours.

Biological Sources

NCBI Organism Classification: Lilium longiflorum
Cell Type: gamete
Cellular Component: pollen wall; exine; intine; sexine; endexine

Biological Context

Biological Process: pollination
Plant Growth: L mature pollen stage

Attribution

Names: Michael Cammer

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35532
Archival Resource Key (ARK): ark:/b7295/w9cil35532

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: multi-photon microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: intrinsic mass distribution
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1024px	0.132µm
Y	1024px	0.132µm
Z	37px	1µm