CIL:35445, Drosophila melanocephala, salivary gland. CIL. Dataset

NCBI Organism:Drosophila melanocephala; Cell Components:polytene chromosome

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35445^* Cite

Description

High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 45 degrees. Grouped with it is an image of the same region taken at a tilt angle of 55 degrees, providing an oblique stereo view of the structure.

Technical Details

Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 0.5 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246

Biological Sources

NCBI Organism Classification: Drosophila melanocephala
Cell Type: salivary gland
Cellular Component: polytene chromosome

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35445
Archival Resource Key (ARK): ark:/b7295/w9cil35445

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: chemically fixed tissue; paraformaldehyde fixation; osmium tetroxide fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5696px	1.5nm
Y	3812px	1.5nm