CIL:35424, Mus musculus, oocyte. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:oocyte Cell Components:microtubule, microtubule organizing center part, nuclear chromosome; Biological process:meiotic spindle organization, microtubule organizing center organization, RNA interference;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35424^* Cite

Description

Time-lapse microscopy of b-Tubulin GFP expressing mouse oocytes injected with siTPX2-5h, a doublestranded siRNA against TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2). Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner. Video S4. Found at: doi:10.1371/journal.pone.0003338.s004

Technical Details

Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: oocyte
Cellular Component: microtubule; microtubule organizing center part; nuclear chromosome

Biological Context

Biological Process: meiotic spindle organization; microtubule organizing center organization; RNA interference
Molecular Function: GTPase activity

Attribution

Names: Stephane Brunet; Julien Dumont; Karen W. Lee; Kazuhisha Kinoshita; Pascale Hikal; Oliver J. Gruss; Bernard Maro; Marie-Helene Verlhac
Pubmed: 18833336
Link: http://www.plosone.org/article/info:doi%2F10....

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35424
Archival Resource Key (ARK): ark:/b7295/w9cil35424

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization Methods: Green fluorescent proteins from Aequorea
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	130px	——
Y	130px	——

Time	1200 microns	39