Freeze substituted Lilium pollen tube immunolabeled for microtubules (blue>magenta) and imaged using laser scanning confocal microscopy. Shown is a projection of x-y slices revealing the distribution of microtubules in the sub-apical region. This group of images also contains an x-z projection of the same specimen.
Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-tubulin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of x-y slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 2001px | —— |
| Y | 424px | —— |
