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NCBI Organism:Homo sapiens; Cell Components:, spindle, condensed chromosome; Biological process:mitosis
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35158*  Cite 
Description

Live mitotic HeLa cell treated with epsin1 siRNA, DiOC6(3)to label mitotic membranes (green), and Hoechst 33258 to label chromosomes (blue). Confocal images were taken at 0.118 μm steps along the Z-axis. Epsin1 depleted cells show aberrant membrane morphologies compared with control cells (see grouped image).

Technical Details

A Leica TCS SP5 DM6000B confocal laser scanning microscope with HCX Plan Apo 63x/1.4 oil objective lens was used to capture the images.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
cervical epithelium
Cell Line
HeLa
Cellular Component
mitotic membranes
spindle
condensed chromosome
Biological Context
Biological Process
mitosis
Attribution
Names
Zhonghua Liu
Yixian Zheng
Published
Liu N, Zheng Y, 2009 J. Cell Biol. 186, 473-480
Pubmed
19704018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35158
Archival Resource Key (ARK)
ark:/b7295/w9cil35158
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
Hoechst 33258
DiOC6 (3,3'-dihexyloxacarbocyanine iodide)
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.03µm
Y 1024px 0.03µm
Z 156px 0.118µm