CIL:35150, Homo sapiens, retinal pigment epithelium. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35150^* Cite

Description

Movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using DIC optics. This movie and the stack of images used to prepare them are grouped in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 100x HCX PLAN APO NA 1.35 objective lens. images were captured at 3 min intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: retinal pigment epithelium
Cell Line: hTERT-RPE1

Biological Context

Biological Process: mitosis; cell proliferation

Attribution

Names: Toshiyuki Hatano; Greenfield Sluder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35150
Archival Resource Key (ARK): ark:/b7295/w9cil35150

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	0.064µm
Y	1024px	0.16µm

Time	3 seconds	1920