CIL:34925, Xenopus laevis, fibroblast. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Types:fibroblast Cell Components:actin cytoskeleton, Arp2/3 protein complex, ; Biological process:, , actin filament organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34925^* Cite

Description

Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 (Arp2/3) primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal Arp2/3 complex at Y-junctions at cell edge. Fluorescence microscopy and corresponding intensity profiles double stained with TRITC-phalloidin (red) and p21 antibodies (green) is available at CIL 34922. Image corresponds to Figure 5d from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: fibroblast
Cellular Component: actin cytoskeleton; Arp2/3 protein complex; cell edge

Biological Context

Biological Process: branching of actin filaments; cytochalasin D treatment; actin filament organization
Molecular Function: structural constituent of cytoskeleton

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34925
Archival Resource Key (ARK): ark:/b7295/w9cil34925

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; platinum replica; primary antibody plus labeled secondary antibody; immuno-gold
Processing History: pseudocolored
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue; methanol fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	780px	2.75nm
Y	659px	2.75nm