CIL:34898. CIL. Dataset

Cell Components:actin cytoskeleton Biological process:, , actin filament organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34898^* Cite

Description

Improved visualization of actin filament branching in lamellipodia. EM of keratocyte or fibroblast lamellipodial actin network after cytochalasin D treatment (0.2 μM for 30 min or 0.5 μM for 10 min). Image corresponds to a panel in Figure 2b from J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 2b are available as CIL 34894-34901.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

Cellular Component: actin cytoskeleton

Biological Context

Biological Process: branching of actin filaments; cytochalasin D treatment; actin filament organization

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34898
Archival Resource Key (ARK): ark:/b7295/w9cil34898

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: shadowing and plating; platinum replica
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue; phalloidin
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	380px	——
Y	313px	——