CIL:34889, fibroblast. CIL. Dataset

Cell Types:fibroblast Cell Components:actin cytoskeleton

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34889^* Cite

Description

Multiple branching of actin filaments in lamellipodia of vertebrate fibroblasts. This image shows a local enlargement of the leading edge shown in overview in CIL 24788. Image corresponds to Figure 1k from J Cell Biol. 1999 May 31;145(5):1009-26. Figures 1i-o correspond to CIL 24888-24893.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

Cell Type: fibroblast
Cellular Component: actin cytoskeleton

Biological Context

Biological Process

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34889
Archival Resource Key (ARK): ark:/b7295/w9cil34889

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: optical path length gradient
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating; platinum replica
Processing History: unprocessed raw data

Sample Preparation

Methods: permeabilized tissue; glutaraldehyde fixed tissue; phalloidin; polyethylene glycol
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	205px	——
Y	213px	——