CIL:34746, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Tetrahymena pyriformis; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus, axonemal microtubule, microtubule basal body, cortical microtubule; Biological process:oral apparatus organization, microtubule basal body organization, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

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Description

The oral apparatus of Tetrahymena. The undulating membrane is composed of two rows of basal bodies but only the outer row is ciliated. Both basal bodies are fully formed but only the ciliated member has an axosome and 9 + 2 axoneme. The oral ribs each contain two microtubular ribbons of 4 and 2 microtubules respectively and alveoli lie between. TEM taken on 8/25/67 by R. Allen with Philips 200 operating at 60kV. Neg. 2,400X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: oral apparatus; axonemal microtubule; microtubule basal body; cortical microtubule

Biological Context

Biological Process: oral apparatus organization; microtubule basal body organization; ciliary axoneme organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34746
Archival Resource Key (ARK): ark:/b7295/w9cil34746

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4900px	6.2nm
Y	6083px	6.2nm