CIL:34736, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Tetrahymena pyriformis; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, cortical cytoskeleton, cortical microtubule cytoskeleton, mitochondrion, coated pit; Biological process:microtubule cytoskeleton organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34736^* Cite

Description

An image of the cortex of Tetrahymena showing a longitudinal microtubular ribbon sandwiched between the alveoli and the epiplasm in the crest of the pellicular ridge. A parasomal sac, the distal end of basal body, the proximal end of cilium, a mucocyst, and mitochondrion are all visible in this micrograph. TEM taken on 1/12/71 by M. Aihara with Hitachi HU11A operating at 75kV. Neg. 17,000X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34727) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; cortical cytoskeleton; cortical microtubule cytoskeleton; mitochondrion; coated pit

Biological Context

Biological Process: microtubule cytoskeleton organization

Attribution

Names: Richard Allen (University of Hawaii); M. Aihara

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34736
Archival Resource Key (ARK): ark:/b7295/w9cil34736

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: intrinsic mass distribution; stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; gold
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2982px	——
Y	3352px	——