CIL:34727, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Tetrahymena pyriformis; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, microtubule basal body, cilium axoneme, mitochondrion, coated pit, cortical microtubule cytoskeleton; Biological process:cortical cytoskeleton organization, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34727^* Cite

Description

A high resolution image of a longitudinal microtubular ribbon sandwiched between the alveoli and the epiplasm in the crest of the pellicular ridge. A parasomal sac, the distal end of basal body, the proximal end of cilium, a mucocyst, and mitochondrion are all visible in this micrograph. TEM taken on 1/12/71 by M. Aihara with Hitachi HU11A operating at 75kV. Neg. 17,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; microtubule basal body; cilium axoneme; mitochondrion; coated pit; cortical microtubule cytoskeleton

Biological Context

Biological Process: cortical cytoskeleton organization; cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii); M. Aihara

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34727
Archival Resource Key (ARK): ark:/b7295/w9cil34727

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4550px	0.88nm
Y	5208px	0.88nm