CIL:34614, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Tetrahymena pyriformis; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, nuclear pore, nuclear membrane; Biological process:macronucleus organization, nuclear membrane organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34614^* Cite

Description

The nuclear envelope of both the macronucleus, seen here, and the micronucleus consists of 2 membranes containing numerous perforations called pores. Proteins are imported through these pores and ribosomal subunits are transported out of the macronucleus through the same pores. TEM taken on 8/16/67 by R. Allen with a Philips 200 operating at 60kV. Neg. 12,400X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34536) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: macronucleus; nuclear pore; nuclear membrane

Biological Context

Biological Process: macronucleus organization; nuclear membrane organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34614
Archival Resource Key (ARK): ark:/b7295/w9cil34614

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: intrinsic mass distribution; stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; gold
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3093px	——
Y	3869px	——