CIL:34602, Opercularia [NCBITaxon:168247], cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Opercularia [NCBITaxon:168247]; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, cell cortex, oral apparatus, cytoplasm, ; Biological process:oral apparatus organization, macronucleus organization, cytoplasm organization, cortical cytoskeleton organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34602^* Cite

Description

Nice view of the oral apparatus, macronuclear organization, cortex, and cytoplasm of Opercularia. Neg of unknown magnification. Estimate sizes from the approximate 0.2µm diameter of a ciliary axoneme. The raw film was scanned with an Epson Perfection V750 Pro.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Opercularia [NCBITaxon:168247]
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component: macronucleus; cell cortex; oral apparatus; cytoplasm; striated fiber

Biological Context

Biological Process: oral apparatus organization; macronucleus organization; cytoplasm organization; cortical cytoskeleton organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34602
Archival Resource Key (ARK): ark:/b7295/w9cil34602

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5834px	——
Y	4488px	——