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Licensing
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34562*  Cite 
Description

Drosophila melanogaster thoracic tergotrochanter muscle from a four week-old wild-type animal. A longitudinal section of myocyte is displayed. Sections of parallel myofibrils are seen with distinguishable sarcoplasmic reticulum.

Technical Details

Tissue was dissected using a freeze-fracture technique, fixed in 2.5% glutaraldehyde in 0.1 M sodium cacadylate buffer, pH 7.4, post-fixed in 2% osmium tetroxide solution, stained in 1% uranyl acetate, and embeddeded in EMbed 812 resin. Sections were post-stained with 2% uranyl acetate and stained with Reynolds’s Lead Citrate. Images were obtained using transmission electron microscopy techniques, employing a Phillips CM-100 transmission electron microscope. Images were taken on Kodak EM 4489 film, scanned with a Microtek ScanMaker i900, and post-processed using Adobe Photoshop CS4. This image was taken at a magnification of 8900x.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
muscle cell
Cellular Component
myofibril
sarcoplasmic reticulum
Biological Context
Biological Process
flight
Attribution
Names
Catherine Braine
Marian Rice
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL34562
Archival Resource Key (ARK)
ark:/b7295/w9cil34562
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
uranyl salt
lead salt
Processing History
digitized film
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in epoxy resin embedment
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1766px 42.33µm
Y 1832px 42.33µm