CIL:34532, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Tetrahymena pyriformis; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cytoplasm, mitochondrion, mitochondrial inner membrane, peroxisome, rough endoplasmic reticulum, polysome; Biological process:cytoplasm organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34532^* Cite

Description

High resolution image of the cytoplasm of Tetrahymena. Mitochondria have tubular cristae, infoldings of their inner membranes. Peroxisomes, with a few tubular inclusions, mucocysts, and a planar view of rough ER, to which many polysomal ribosome arrays are attached, fill the cytosol. TEM taken on 2/11/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Tetrahymena pyriformis
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cytoplasm; mitochondrion; mitochondrial inner membrane; peroxisome; rough endoplasmic reticulum; polysome

Biological Context

Biological Process: cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34532
Archival Resource Key (ARK): ark:/b7295/w9cil34532

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4687px	0.77nm
Y	5356px	0.77nm