CIL:34524, Homo sapiens, sperm. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:sperm Cell Components:flagellum Biological process:ciliary or flagellar motility, sperm motility;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34524^* Cite

Description

Human sperm diluted in buffer imaged at room temperature within two hours of ejaculation. Cell at center swims at constant speed.

Technical Details

Sperm were diluted with PBS and plated in a coverslip bottom chamber on an Olympus IX70 with 60X N.A. 1.4 phase 3 optics. Images were collected with an air cooled CCD camera (either Roper Coolsnap or Cooke Sensicam) at 12 bits and converted to 8 bits. The time interval was less than 1 second per image.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: sperm
Cell Line: Mature sperm cell
Cellular Component: flagellum

Biological Context

Biological Process: ciliary or flagellar motility; sperm motility
Human Development Anatomy: gonadal component

Attribution

Names: Michael Crammer

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL34524
Archival Resource Key (ARK): ark:/b7295/w9cil34524

Imaging

Image Type: recorded image
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: living tissue
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	640px	0.22µm
Y	480px	0.22µm