This video shows a typical N. gonorrhoeae microcolony interacting with the tips of our micropillar force sensing device. The video is speeded 10 times (×10) and the pilars are 3 micron apart center to center. A pulling force exerted on a pillar, such as that imposed by an attached pilus fiber, causes a displacement of the pillar tip. The displacement can be correlated to the force applied on the pillar after proper calibrations. Video corresponds to Video 1 from PLoS Biol. 2008 Apr 15;6(4):e87.