CIL:31927, Homo sapiens, retinal pigment epithelium. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:31927^* Cite

Description

Time lapse movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. The time series of images used to prepare the movie is grouped with it in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: retinal pigment epithelium
Cell Line: hTERT-RPE1

Biological Context

Biological Process: mitosis; cell proliferation

Attribution

Names: Toshiyuki Hatano; Greenfield Sluder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL31927
Archival Resource Key (ARK): ark:/b7295/w9cil31927

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	0.322µm
Y	1024px	0.322µm

Time	180 seconds	486