CIL:2882, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Coleps hirtus; Cell Types:eukaryotic cell, , ; Cell Components:microtubule associated complex, ; Biological process:microtubule cytoskeleton organization, microtubule bundle formation, microtubule-based process;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:2882^* Cite

Description

A high resolution image of a bundle of microtubules supporting the oral apparatus of Coleps. This micrograph shows the detail of microtubular cross-bridging within one of these rods. Microfibrillar bridges are located at 22nm intervals within the hexagonally packed microtubules. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/29/69 by R. Allen with Philips 300 operating at 60kV. Neg. 63,750X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: microtubule associated complex; microtubular crossbridging

Biological Context

Biological Process: microtubule cytoskeleton organization; microtubule bundle formation; microtubule-based process
Molecular Function: microtubule binding

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL2882
Archival Resource Key (ARK): ark:/b7295/w9cil2882

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4039px	0.3nm
Y	2798px	0.3nm