Z-stack from rat brain cortex showing a subset of fluorescent-protein expressing neurons and microglia. Most prominent in this stack is an aggregation of microglial cells surrounded by axons and dendrites coursing though the tissue section. This stack is accompanied by a colorized maximum projection image in this image group. Neurons are expressing soluble YFP using the neuron-specific TH1 promoter. Microglia are expressing soluble GFP (transgene inserted in the Cx3cr1 locus). The z-stack was acquired with an iMIC Andromeda laser spinning disc confocal microscope (http://www.till-photonics.com/Products/andromeda.php), using an Olympus UPlanSApo 60X/NA1.35 objective, and: Andor Clara (Andor) CCD Camera. Both fluorophores were excited with a 488nm laser, and so are not distinguished from each other, with these filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma); Emission, FF01-390/482/563/640 (Semrock). .